武 汉 和 视 光 声 科 技 有 限 公 司
CalPACT / Union Photoacoustic Technologies

单脉冲小动物全身光声成像系统(SIP-PACT)

SIP-PACT是武汉和视光声制造的用于小动物活体全身成像的商业化产品。小动物的成像在临床前研究中发挥了不可或缺的作用,提供了与临床相关的高维生理、病理和表型认知。然而纯光学成像受限于成像深度(只有1-2毫米)或很低的深度分辨率比(1/3);用于小动物全身或全脑成像的非光学技术亦缺乏时空分辨率或功能对比。SIP-PACT通过高时空分辨率(125 μm 平面分辨率,50 μs /帧的数据采集和50 Hz 帧频),深层组织成像(体内48毫米横截面宽度)、结构上的、动态和功能的对比,和全视图的保真度减轻了这些限制。SIP-PACT实现了小动物活体全身或全脑动态图像,实时获取清晰的亚器官的结构和功能细节。SIP-PACT还能追踪未标记的循环黑色素瘤细胞, 并对整个成年老鼠大脑的血管和功能连接进行了成像。

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SIP-PAC技术优点
  • 功能成像,包括血管生成、缺氧、高氧、血流等

  • 无痛成像

  • 快速成像

  • 分子成像,包括蛋白质、染料、药物、纳米颗粒等

  • 高空间分辨率

  • 100%安全

  • 无需造影剂配合(CE-MRI需要)

  • 没有电离辐射(钼靶有)


产品参数
  • 全身小动物成像,单向穿透深度:4.8 cm 的横截面宽度

  • 空间分辨率:125 μm

  • 2D成像时间分辨率:10 Hz 可升级到50 Hz

  • 激光曝光:<100 mJ/cm2 (符合ANSI在1064 nm 波长的安全限制)

  • 激光能量:>1 焦耳/脉冲 (分布于组织表面)


活体小鼠二维光声动态成像
  • 肝脏










  • 脾脏




  • 心脏和肺





  • 左肾和右肾




血氧饱和度测试
  • 小鼠氧气挑战实验

     





肿瘤细胞追踪
  • 小鼠脑部循环肿瘤细胞的无标记追踪



      

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视频



   

Supplementary Video 1

In vivo label-free PACT imaging of mouse internal organs. The scanning red line shows the corresponding elevational position of the cross-sectional image. The red box indicates the position of the close-up image. The light fluence on the animal skin was 18 mJ/cm2 at 1064 nm, with a pulse repetition rate of 50 Hz. Stepping along the animal trunk with a step size of 0.08 mm, a total of 600 cross-sectional images were acquired, with a 25 mm by 30 mm cm field of view.




Supplementary Video 2

In vivo label-free PACT imaging of mouse whole-body anatomy at a cross-section of the upper thoracic cavity, with contrast enhancement filtering described in Online Methods. The light fluence on the animal skin was 18 mJ/cm2 at 1064 nm, with a pulse repetition rate of 50 Hz.




Supplementary Video 3

In vivo label-free PACT imaging of mouse whole-body anatomy at a cross-section of the lower thoracic cavity, with contrast enhancement filtering described in Online Methods. The light fluence on the animal skin was 18 mJ/cm2 at 1064 nm, with a pulse repetition rate of 50 Hz.




Supplementary Video 4

In vivo label-free PACT imaging of mouse whole-body anatomy at a cross-section of the liver, with contrast enhancement filtering described in Online Methods. The light fluence on the animal skin was 18 mJ/cm2 at 1064 nm, with a pulse repetition rate of 50 Hz.




Supplementary Video 5

In vivo label-free PACT imaging of mouse whole-body anatomy at a cross-section of the upper abdominal cavity, with contrast enhancement filtering described in Online Methods. The light fluence on the animal skin was 18 mJ/cm2 at 1064 nm, with a pulse repetition rate of 50 Hz.




Supplementary Video 6

In vivo label-free PACT imaging of mouse whole-body anatomy at a cross-section of the lower abdominal cavity, with contrast enhancement filtering described in Online Methods. The light fluence on the animal skin was 18 mJ/cm2 at 1064 nm, with a pulse repetition rate of 50 Hz.




Supplementary Video 7

In vivo PACT mouse liver cross-sectional images reconstructed from increasing angular coverage. Angle was gradually increased from 45 to 360 degrees. Reconstruction artifacts are significantly mitigated while angular coverage increases.



Supplementary Video 8

Pulse wave induced cross-sectional area changes of two vertical arteries over time. The right panel co-plots the normalized cross-sectional areas of the two arteries and shows the relatively stable phase delay between them.






Supplementary Video 9

In vivo label-free PACT imaging of mouse brain response to oxygen challenge. During the measurement, the pulse repetition rate was 10 Hz. The movie was created by down sampling at a ratio of 25:1.





Supplementary Video 10

Lower abdominal cavity oxygenation response of a mouse during whole-body oxygen challenge. This cross section shows the spleen, cecum, intestine, and both kidneys. The bottom-right panel shows the change of the signal level averaged over the entire FOV.





Supplementary Video 11

Label-free tracking of circulating melanoma tumor cells in the mouse brain in vivo. The light fluence on the animal skin was 8 mJ/cm2 at 680 nm, with a pulse repetition rate of 10 Hz.



Supplementary Video 12

In vivo monitoring of dye perfusion in the mouse brain. The right panel shows the normalized change of the signal level averaged over the entire FOV. The dye solution (100 µL with 0.5% mass concentration) was injected through the carotid artery. The light fluence on the animal skin was 18 mJ/cm2 at 1064 nm, with a pulse repetition rate of 50 Hz.

参考
[Li, L.; Zhu, L.; Ma, C.; Lin, L.]; Yao, J.; Wang, L.; Maslov, K.; Zhang, R.; Chen, W.; Shi, J. H.; Wang, L. V.; "Single-impulse panoramic photoacoustic computed tomography of small-animal whole-body dynamics at high spatiotemporal resolution," Nature Biomedical Engineering 1 0071 (2017)
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